Surg Cosmet Dermatol. 2018;10(4):321-324
INTRODUCTION: The interaction between the skin and air pollutants has demonstrated effects in the cutaneous barrier, as well as triggering oxidative processes related to premature ageing of the skin. The aryl hydrocarbon receptor (ArH) is a transcription protein that interacts with xenobiotics, regulating the transcription of genes involved with oxidative stress, inflammation, immunosuppression and pigmentation, besides leading to processes related to ageing and carcinogenesis.
OBJECTIVE: Evaluate the anti-pollution efficacy of an antioxidant association for the prevention of the nuclear translocation of the AhR receptor.
METHODS: A in vitro model (keratinocyte culture) was exposed to cigarette smoke and the presence of AhR was measured through sanduwich ELISA assay.
RESULTS: The treated culture demonstrated inhibition of the nuclear translocation of AhR in all concentrations evaluated: ArH increase of 75.38%; 59.88% and 117.79% are seen with the concentrations of 0.316; 0.100 and 0.0316mg / mL, respectively.
CONCLUSION: The results suggest the ability of the formulation analyzed in preventing the activation of genes responsible for the damaging effects of cigarette smoke.
Keywords: DNA Damage; Air Pollution; Aryl Hydrocarbon Receptor Nuclear Translocator