Introduction: Skin aging is a complex biological process that is characterized not only by clinical but also cellular alterations. The use of effective topical formulations for reducing and preventing oxidative stress is of utmost importance to delay cellular senescence. Objective: To evaluate the in vitro and in vivo efficacy of a formulation containing vitamin C, fragmented hyaluronic acid and mannose, in the prevention of skin aging. Methods: Clinical, subjective, and instrumental evaluations were carried out in 37 women (mean age: 46 years) and in vitro comparative evaluations of a dermal equivalent model contraction. Results: Statistical improvement was observed in the clinical parameters of hydration, sagginess, brightness, uniformity of skin color and amount of wrinkles after 3 months of treatment. A reduction by 12.6% in the volume of wrinkles 30 minutes after application and by 17.8% after three months of use was also evidenced by instrumental evaluation. There was greater contraction of the dermal equivalent model when compared to a commercial product for wrinkle reduction containing the same active principle. Conclusions: The product was proven effective in skin rejuvenation, by significantly reducing the amount of wrinkles. The in vitro study showed an increase in collagen synthesis, suggested by the greater activation of fibroblasts in the dermal equivalent model.

Keywords: SKIN AGING; ASCORBIC ACID; HYALURONIC ACID; MANNOSE