Evaluation of the moisturizing and protective activity of a topical product containing saccharide isomerate and hydroxyethyl urea, on the skin barrier

Abstract

INTRODUCTION: The proteins involved in the process of epidermal differentiation play a key role in retention of water and maintenance of the stratum corneum.
OBJECTIVE: To assess the compaction of the skin barrier and protein expression of filaggrin, involucrin, loricrin and sphingomyelin after the use of a moisturizing cream containing saccharide isomerate and hydroxyethyl urea.
MATERIALS AND METHODS: Ex vivo evaluations were performed in skin culture in addition to immunofluorescence in order to identify proteins, as well as in vitro analysis with tissue culture in culture medium and histological sections, all of which compared with a control group.
RESULTS: In the ex vivo evaluation, the protein markers increased in the group treated with the moisturizing cream as compared to the skin culture model with atopic dermatitis. In the in vitro evaluation, it was possible to observe an improvement in the skin barrier compaction with the application of the cream on a reconstituted three-dimensional epidermis model, as compared with the control.
CONCLUSIONS: The moisturizing cream promoted a significant increase in the production of filaggrin, involucrin and loricrin, as well as in the compaction of the skin barrier, thus suggesting a positive effect on skin hydration and protection of the cutaneous barrier, preserving and restoring the skin.

Keywords: Administration; Amino acids; Carbohydrates; Cutaneous; Fluid therapy; Keratins; Keratinocytes; Skin; Skin care; Skin cream; Peptides; Proteins; Urea; Wetting agents

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