Introduction: Photodynamic therapy destroys target cells by directly harming them, injuring the vascular stroma, and activating the immune system.
Objective: To study photoaged skin''''''''s immune response to photodynamic therapy.
Methods: Thirteen female patients underwent three treatment sessions with delta 5-aminolevulinic acid combined with 630 nm diode light. The immunohistochemical response of the skin''''''''s immune system was evaluated using the markers anti-CD1a, anti-CD4+, anti-CD8+, anti-TNFa, anti-IFNy and anti-IL4 before, 24 hours after the first session, and 21 days after the third session.
Results: At the final evaluation, patients demonstrated a decrease in CD4 and CD8 lymphocytes, an increase in interleukin-4 in the epidermis and dermis, and a reduction and an increase of TNF-a in the epidermis and dermis, respectively. The Langerhans cell and INF-y populations remained unchanged.
Conclusion: Regarding the immune system, photodynamic therapy did not cause changes in Langerhans cells; therefore, there was little local immunosuppression. CD4 and CD8 lymphocyte apoptosis was observed, suggesting that photodynamic therapy can be useful in treating inflammatory diseases. The changes in TNF-a and an increase of IL4 in the dermis and epidermis suggest a humoral type response, which was also evidenced by the absence of INFy.

Keywords: PHOTOCHEMOTHERAPY, AMINOLEVULINIC ACID , SKIN AGING

Introduction: Introduction: Mineral water has been used in Dermatology for its moisturizing and anti-inflammatory effects. Its penetration seems to be superficial. An elastic liposome containing mineral water was developed in efforts to improve its penetration and action in the skin.
Objectives: This study evaluated the efficacy of a 30-100 nm liposome, made of trehalose and soy phosphatidylcholine, which can cross 30 nm spaces and deliver the mineral water to deeper layers of the skin.
Methods: The RAMAN method was used to evaluate the permeation of the liposome into the skin. Its moisturizing effects were measured through the genic expression of filaggrin and acquaporin, and its anti-inflammatory effects were measured through NF-¿ß quantities.
Results: After 30 minutes, the phosphatidylcholine was localized 1-7 nm deeper in the corneum layer; 60 minutes after, the liposome had penetrated to the spinous layer; and no changes were verified after 120 minutes. The genic expression of filaggrin increased 3 times and the expression of acquaporin increased 1.8 times. NF-¿ß activity decreased 47%.
Conclusion: Nanotechnology and its benefits are very important to dermatology. The development of liposomes with Brazilian technology demonstrates the emergence of a knowledge base regarding cosmetic delivery systems in the country. Therefore, Brazil''''''''s wealth of mineral spring waters represents a considerably valuable resource and should be the object of further studies in dermatology. This study has verified that liposome-encapsulated water can permeate the skin and provide enhanced biological effects.

Keywords: MINERAL WATERS, LIPOSOMES, SKIN, COSMETICS, AMBULATORY SURGICAL PROCEDURES, SURGICAL PROCEDURES, MINOR